DES and astrocytoma (excluding glioblastoma): Importantly, filaments of glial fibrillary acidic protein (GFAP), another type III intermediate filament protein, also adopted a peripheral distribution in mitotic U-251 MG astrocytoma cells, whereas desmin filaments disassembled in mitotic undifferentiated C2C12 myoblasts, showing a predominantly diffuse cytoplasmic staining (Fig. 4b).