Accordingly, dividing SW13/cl.2 cells, which are nestin-negative, show persisting filaments, nestin-positive MCF7 cells54 display vimentin disassembly, and other cell types expressing variable nestin:vimentin proportions, such as U-251 MG astrocytoma cells33 or BAEC55, show mixed patterns. This evidence concerns the gene VIM and astrocytoma (excluding glioblastoma).