Besides, researchers also used transcription activator-like effector nucleases (TALENs) to generate mutations in the irf8 gene in zebrafish, which – as in mice – causes a type of MPN known as chronic myeloid leukemia (CML)-like disease (Holtschke et al., 1996; Zhao et al., 2018). This evidence concerns the gene IRF8 and chronic myelogenous leukemia, BCR-ABL1 positive.