Given the strong correlation between alterations of SPINT1 levels with the progression of SKCM and the crosstalk with the tumor immune microenvironment, we crossed the zebrafish line kita:Gal4;HRAS-G12V, which expresses the human oncogene HRAS-G12V in melanocytes and spontaneously develops SKCM [8], with the zebrafish mutant line spint1ahi2217Tg/hi2217Tg [19], which presents chronic skin inflammation (Fig. 3a). This evidence concerns the gene HRAS and neoplasm.