Q-pcr assays also indicated that some certain stemness associated genes, such as SOX2 [24], OCT3/4 [25], NOTCH1 [26], C-MYC [27] and ABCG2 [28] were decreased concurrently with NAP1L1 down-regulation in HCC cells. This evidence concerns the gene NOTCH1 and hepatocellular carcinoma.