AR and Familial prostate cancer: Three established prostate cancer cell lines were chosen for method establishment, each cell line representing a specific status of AR-full length (AR-FL) and ARV7 protein expression: 22Rv1 (AR-FL+/AR-V7+), LNCaP (AR-FL+/AR-V7+/−), and PC3 (AR-FL−/AR-V7−).