Analysis of RNA-seq dataset from spleens of chickens four days after intranasal infection with bursal disease virus (IBDV) showed a significant induction of TNF and TNFR1 (p ≤ 0.05); no induction of TNFR2; and no or low expression of leptin and LEPR (<1 RPKM), respectively (Figure 3A). This evidence concerns the gene TNF and infection.