We investigated also the effects of two high affinity variants obtained from PD-L1_1 (Cembrola et al., Rapid affinity maturation of novel anti-PD-L1 antibodies by a fast drop of the antigen concentration and FACS selection of yeast libraries, submitted for publication, 2019), and we found that they inhibited tumor cell viability more efficiently than the parental PD-L1_1. The gene discussed is CD274; the disease is neoplasm.