AGT and cardiac hypertrophy: To test the role of ABCB7 in cardiac hypertrophy, we transfected H9C2 cells with ABCB7- targeting siRNA to downregulate ABCB7 or with flag-ABCB7 to induce ABCB7 overexpression and then stimulated the cells with angiotensin II to induce hypertrophy (Fig. 3D and Supplementary Fig. S5C,D).