Through ATAC-seq technique, we found that some gene loci of upregulated gene expression in PTL-CAR-T cells from xenograft-derived tumor, incliuding ifng (Chr12: 68,154,768-68,159,747), tnfa (Chr6: 31,575,567-31,578,336), and cxcl12 (Chr10: 44,370,165-44,386,493), bcl2 (Chr18: 63,123,346-63,320,128), and CD56 (Chr11: 112,961,247-113,278,436), exhibited much higher peaks, indicating that the chromatin accessibility of many functional genes had significantly changed (Fig. 3d). Here, CXCL12 is linked to neoplasm.