TWIST1 and hepatocellular carcinoma: Moreover, given that an abnormal Warburg effect in malignant cells may alter the EMT, which induces the resistance of HCC cells to antitumor chemotherapies, we tested EMT marker genes Twist, Snail, N-cadherin, and Vimentin, and found that they were downregulated after Betulin treatment, indicating a correlation between SREBP-1 and chemoresistance.