After establishing a stable overexpression system of mimic miR-145-5p through the lentiviral transfection of G15 cells (Fig. 7k), we determined that the sphere percentage and the levels of Nestin, CD133, SOX2, and SOX9, as well as tumor sphere percentage were markedly decreased in the mimic miR-145-5p group compared with the NC group (Fig. 7l-o). This evidence concerns the gene SOX2 and neoplasm.