To determine whether this circuit linking Wnt5a with NF-κB is prevalent in melanoma, we performed a similar Wnt5a treatment in four additional melanoma cell lines derived from metastasis (A375, WM9, SK-Mel28, and SK-Mel2), a cell line derived from a primary tumor (WM983A), and a melanocyte cell line. Here, WNT5A is linked to melanoma.