IL-36γ has been shown to be important for formation and maturation of TLS in a murine model of DC-induced CLR; in human CRC, IL-36 is secreted predominantly by M1 macrophages and the vasculature, and is associated with a CD4+ central memory T-cell infiltrate and an increased density of B cells in CLR (29, 40). This evidence concerns the gene DCLK3 and colorectal carcinoma.