CHAT and Anxiety: Our results also reveal ChAT::Cre+ rats to be anxiolytic, as was assessed in three frequently used tests of anxiety-like behavior, with such tests that consistently demonstrate the ability to reveal anxiolytic or anxiogenic profiles of rodents using either pharmacological- or transgenic manipulations (Crawley 1985; Belzung and Griebel 2001; Prut and Belzung 2003).