In vivo validation of splice modulating AO-mediated down-regulation of ITGA4 activity was performed in the experimental autoimmune encephalomyelitis (EAE)17 mouse model of MS, by injecting peptide conjugated phosphorodiamidate morpholino oligomers (PPMOs) that were designed to induce specific Itga4 exon skipping, into mice with chronic EAE. Here, ITGA4 is linked to myeloid sarcoma.