Therefore, this study aimed to explore the individual response pattern of CCA patients to gemcitabine, cisplatin and gemcitabine plus cisplatin using the HDRA method, as well as exploring the expression of DCK, hENT-1, RRM1, and MT and ERCC1 in the tissues of CCA patients in the same cases in a prospective study. This evidence concerns the gene DCK and cholangiocarcinoma.