Thus, we next investigated the change of SASP HCC cellular senescence induced by miat. We detected the significant induction of major pro-inflammatory cytokines, such as tnf-α, il-1α, il-1β, il-6, and il-8 in miat silenced HepG2 cell lines and found that miat silencing promotes the secretion of SASP in HCC cell lines (Figure 6Q and Supplementary Figure 8O). This evidence concerns the gene TNF and hepatocellular carcinoma.