SIRT1 and hepatocellular carcinoma: Finally, we detected the ability of cell proliferation in HCC cell lines and verified that sh-miat /miR-22-3p/sh-sirt1 silencing inhibited HCC cell proliferation, and these tumor suppressive effects were significantly reversed after miR-22-3p KD or sirt1 OE in both HCC cell lines (Figure 6T and Supplementary Figure 8R).