We also detected the expression of il-6 in HepG2 and SMMC-7721 cells with different treatments as described in Figure 6F or Supplementary Figure 8S, and obtained a similar result that sh- miat /miR-22-3p/sh-sirt1 promoted the secretion of SASP in HCC cell lines, and miR-22-3p inhibition or sirt1 overexpression reversed the SASP generation induced by miat silencing (Figure 6S and Supplementary Figure 8Q). Here, SIRT1 is linked to hepatocellular carcinoma.