In addition, sirt1 overexpression rescued miR-22-3p (Figure 5J) induced inhibition of cell proliferation (Figure 5K), colony formation (Figure 5L), migration (Figure 5M, 5N) and EMT transition (Figure 5O) in HepG2 cells, while sirt1 knockdown restored the pro-tumorigenic effects of HCC inhibited by miR-22 inhibitor (sh-miR-22) (Supplementary Figure 6G–6P). This evidence concerns the gene SIRT1 and hepatocellular carcinoma.