Here, we investigated the effects of doxorubicin-loaded HSA nanoparticles on the viability of the neuroblastoma cell line UKF-NB-3 and its sub-lines adapted to doxorubicin (UKF-NB-3rDOX20) and vincristine (UKF-NB-3rVCR1), which both display ABCB1 activity and resistance to doxorubicin. This evidence concerns the gene ABCB1 and neuroblastoma.