TLR7 and rheumatoid arthritis: FISH was performed with two X Chr-specific probes (X centromere and TLR7) and one Y centromere-specific probe on peripheral blood cells in metaphase from 14 men with RA (5 with normal CN and 9 with high CN) and 11 healthy men (6 with normal CN and 5 with high CN) (Fig. 6).