The experimental results showed that: after PTEN gene silencing, compared with the untransfected and control siRNA groups and positive control β-actin (Fig. 3a), the expressions of PTEN proteins in four colon cancer cells were significantly inhibited (P < 0.01, respectively, compared with the untransfected and control siRNA groups), and the experiment showed that PTEN siRNA primer design and cell transfection were successful (Fig. 3b). Here, PTEN is linked to malignant colon neoplasm.