ACTB and malignant colon neoplasm: The experimental results showed that: after PTEN gene silencing, compared with the untransfected and control siRNA groups and positive control β-actin (Fig. 3a), the expressions of PTEN proteins in four colon cancer cells were significantly inhibited (P < 0.01, respectively, compared with the untransfected and control siRNA groups), and the experiment showed that PTEN siRNA primer design and cell transfection were successful (Fig. 3b).