Major downstream pathways of AMPK, such as the sterol regulatory element-binding transcription factor 1 (SREBF1, also known as SREBP1) pathway related to lipogenesis and the mechanistic target of rapamycin (mTOR) pathway, whose activation contributes to obesity, were downregulated by GTE (Figure 3b). This evidence concerns the gene PRKAA2 and obesity due to melanocortin 4 receptor deficiency.