In this study, we found that the induction of CTSD in L1-expressing CRC cells involves Wnt/β-catenin signaling, since by stimulating this pathway with the GSK3 inhibitor LiCl, we observed an increase in CTSD expression, while suppressing NF-κB signaling had no effect on CTSD levels. The gene discussed is NFKB1; the disease is colorectal carcinoma.