INS and Impaired glucose tolerance: Figure 3 shows that an absolute 17 h fast in AL animals caused an impaired glucose tolerance at t = 5 min (Figure 3A) and an increased net AUC (Figure 3B). Finally, the 17 h absolute-fasted, AL and TRF animals had a smaller insulin response as compared to the relative fasted AL animals as well as a smaller net AUC (Figures 3C,D).