In the present study, we investigated the effects of TDRG1 protein on tumour growth in vivo and on autophagy, cell viability, apoptosis and the p110β/Rab5/Vps34 (PI3‐kinase Class III) pathway of seminoma TCam‐2 cells in vitro, with or without CDDP treatment. The gene discussed is TDRG1; the disease is seminoma.