Taken together, the presented data not only demonstrate the practicality of using FFPE samples for robust PCa biomarker discovery, more importantly, it also identified a panel of protein biomarker candidates for PCa diagnosis, among which MDH2, TPP1, and ACAD8 were most significant regardless of tissue formats (fresh or FFPE, punch or micrometer section) and patient populations. Here, ACAD8 is linked to posterior cortical atrophy.