To dissect the effect of SATB2-AS1 in CRC, we knockdown SATB2-AS1 using two chemically modified siRNAs in HCT-116 and HCT-8 cells, and the efficiencies of interference were confirmed by qRT-PCR (Additional file 3: Figure S2a). The gene discussed is SATB2; the disease is colorectal carcinoma.