To further investigate the biological functions of the α7-nAChR in HCC using scrambled control and α7-nAChR-KD Hep-J5 and Mahlavu cells, we demonstrated that loss of α7-nAChR expression significantly diminished HCC cell viability as expressed by 41% (p < 0.05) and 60% (p < 0.01) reductions in the population of viable Hep-J5 cells after 24 and 48 h, respectively (Figure 3A). Here, CHRNA7 is linked to hepatocellular carcinoma.