To examine the effect of the α7-nAChR on the invasive ability of HCC cells, we performed invasion assays and found that knockdown of α7-nAChR significantly reduced the number of invading Hep-J5 and Mahlavu α7-nAChR-KD cells compared to scrambled control cells (Figure 3G). The gene discussed is CHRNA7; the disease is hepatocellular carcinoma.