Immunohistochemical labeling for Iba-1 of elephant tissues that died in association with EEHV1A-HD, EEHV4-HD, or co-infections of EEHV1A and 4-HD indicated a significant increase in Iba-1 positive cells in the hearts, livers, small intestines and lymph nodes, when compared to those in the EEHV-negative control (Fig 2A and 2B). The gene discussed is AIF1; the disease is coinfection.