ANO1 and cancer: ANO1 gene amplification or mutation is not clear in ATC, but the amplification of chromosomal loci containing the gene encoding ANO1 (11q13 region) is frequently found in various human cancers such as breast, urinary bladder, prostate, and lung cancer; head and neck squamous cell carcinoma (HNSCC); and glioblastoma (Proctor et al.