1991; Liu et al. 2012; Jia et al. 2015). It has been reported that patients with HNSCC malignancies overexpressing ANO1 have worse prognosis (Duvvuri et al. 2012). Our observation of enhanced ANO1 expression in one ATC tissue sample is obviously too preliminary to conclude the importance of ANO1 in ATC. However, combined with evidence from other cancers, we hypothesized that ANO1 plays a significant role in ATC metastasis, and we decided to explore the potential role of ANO1 in ATC in vitro. Here, ANO1 is linked to head and neck squamous cell carcinoma.