An HLA‐G antibody conjugated to the surface of methotrexate (MTX)‐loaded nanobubbles (mAbHLA‐G/MTX/PLGA NBs) could be specifically transported to the HLA‐G‐positive choriocarcinoma cancer cells JEG‐3 in vitro and tumor tissues in vivo in a tumor‐bearing murine model of BALB/c (nu/nu) nude mice established with JEG‐3 cells. The gene discussed is HLA-G; the disease is neoplasm.