Ultrasonography (US) is the most commonly used imaging examinations with relative low cost, but has low sensitivity (63%) in early detection of HCC.4 Serolgocial test for α-fetoprotein (AFP), AFP-L3, des-gamma-carboxy prothrombin (DCP) have been investigated for HCC diagnosis alone or in combine. The gene discussed is AFP; the disease is hepatocellular carcinoma.