As in this study, Salama et al. did not observe a significant relationship between FOXP3 + cells and MSI,25 and Le Gouvello et al. found a lower mRNA expression level of FOXP3 in MSI-H tumour tissues.47 In CRC, the local infiltration of CD4 + and FOXP3 + cells may be affected by colonic microbiota, rather than by neoantigens. Here, CD4 is linked to neoplasm.