Most notably, the unique enriched motifs at sarcoma-specific binding sites often included motifs of neural crest-related TFs, such as Ebf1, Sox10, and Nur77 (Nr4a1), while those at MEF-specific binding sites included motifs of mesenchymal cell/connective tissue/embryonic tissue-related TFs, such as Runx2, Tead4, and Meis1 (Table 2)30–32. This evidence concerns the gene NR4A1 and sarcoma.