Therefore, we attempted to induce silencing at sarcoma-specific EWS/ATF1 binding sites in sarcoma cells by overexpressing KRAB-fused Ebf1, since the Ebf1 motif is the most abundant motif among sarcoma-specific motifs for EWS/ATF1 binding (Table 2). The gene discussed is EBF1; the disease is sarcoma.