PKD1 and autosomal dominant polycystic kidney disease: The Toronto Genetic Epidemiology Study of PKD (TGESP), a clinically ascertained cohort including primarily ADPKD patients of European descent, identified 60% (131 of 220 families) with PKD1 mutations and 26% (57 of 220 families) with PKD2 mutations using long-range PCR and Sanger sequencing [32].