When using GEPIA to analyze the correlation between hub gene expression and the prognosis of HNSCC, we found that the overall survival rate of high-expressing TGFBI was lower than that of low-expressing TGFBI (p=0.013); compared with low-expressing SPP1, the overall survival rate of high-expressing SPP1 was lower (p=0.045); compared with low-expressing LAMB3, the overall survival rate of high-expression group of LAMB3 was lower (p=0.011). This evidence concerns the gene SPP1 and head and neck squamous cell carcinoma.