In the present work we have demonstrated (i) the technical possibilities for long-term longitudinal DTI analyses in murine models of neurodegenerative diseases, specifically in an ALS model using ultrahigh field (11.7 T) with 30 gradient directions, (ii) the appearance over time of FA abnormalities in the motor cortex as well cingulate, insular and retrosplenial cortices of mice with mutant TDP-43 overexpression in neurons (Thy1.2 promoter) mice, and (iii) the dissociation between pathologic values of diffusivity indexes in motor cortex and normal values in the CST. Here, TARDBP is linked to neurodegenerative disease.