Using the fully automated Elecsys® sFlt-1 and PlGF assays (Roche Diagnostics) for maternal blood testing, cut-offs for the sFlt-1/PlGF ratio were derived and validated to rule out (for up to 4 weeks; sFlt-1/PlGF ratio ≤ 38) or rule in (within 4 weeks; sFlt-1/PlGF ratio > 38) the occurrence of preeclampsia [10, 13]. The gene discussed is PGF; the disease is preeclampsia.