Under these heat-shock mimicking conditions, OLF is in an early unfolding regime that mimics the partially folded state of glaucoma-associated OLF variants, and end-point aggregates have been vetted extensively as amyloid-like35; destabilized OLF variants and full-length myocilin (Fig. 1B) are not available in preparative yields required for the assay. Here, MYOC is linked to glaucoma.