Finally, in support of the hypothesis that Grp94 conformational states are relevant to the aberrant interaction with OLF, we also tested two glaucoma-causing variants OLFD380A or OLFI499F for their rate enhancement with Grp94N (data not shown), experiments that had to be conducted at 32 °C and 28 °C, respectively to match the aggregation kinetics of wild-type OLF at 42 °C. This evidence concerns the gene HSP90B1 and glaucoma.