To further bracket the strength of interaction between Grp94 and OLF, fluorescence polarization experiments were performed with OLFd containing the glaucoma-associated D380A variant (OLFdD380A), a less stable (Tm = 46.6 °C versus for 52.2 °C wild-type OLF) protein with no central Ca2+ 13,40. This evidence concerns the gene HSP90B1 and glaucoma.