INS and fatty liver disease: Fat accumulation in the liver of these animals is related to high cholesterol consumption, as this overload in the cells alters the cholesterol homeostasis.26 In addition, the accumulation of intermediate lipid metabolites, such as diacylglycerol and acylcarnitines, is associated with inflammation and insulin resistance.27,28 A previous study suggested that high dietary cholesterol is a critical factor for the progression of hepatic steatosis and inflammation in animal models.