To further exclude the possibility that Tpl2 may directly function in CD4+ T cell to regulate FH pathogenesis, we adoptively transferred WT or Tpl2-deficient CD4+ T cells into T cell-deficient Rag1-KO mice, which were then induced the FH model by injecting P. acnes/LPS. This evidence concerns the gene RAG1 and familial hyperaldosteronism.