However, AT3 breast tumors were primarily resistant to mAb, exhibiting no benefit in tumor control or overall survival (Fig. 1d; Supplementary information, Fig. S1d).33 Whilst tumor growth in the sensitive (MCA205WT) model displayed a rebound after the period of active anti-PD-1 therapy, we showed that prolonging the administration of anti-PD-1 mAb from 4 to 6 doses failed to extend the efficacy of therapy, culminating in overt tumor progression in the majority of animals (Fig. 1e, f; Supplementary information, Fig. S1f). This evidence concerns the gene PDCD1 and breast neoplasm.