The results showed that YBX1 or G3BP1 overexpression significantly (p < 0.01) promoted tumor cell migration (Fig. 4a and b) and invasion in RCC ACHN cells, while SPP1 depletion strongly attenuated the effect of YBX1 or G3BP1 induced RCC cells migration and invasion (Fig. 4a and b). This evidence concerns the gene SPP1 and renal cell carcinoma.