The results showed that YBX1 or G3BP1 overexpression significantly (p < 0.01) promoted tumor cell migration (Fig. 4a and b) and invasion in RCC ACHN cells, while SPP1 depletion strongly attenuated the effect of YBX1 or G3BP1 induced RCC cells migration and invasion (Fig. 4a and b). The gene discussed is G3BP1; the disease is renal cell carcinoma.