TFRC and hepatocellular carcinoma: Thus to verify how didox modifies the iron status of the HCC cells in comparison with DFP and DFO, HA22T/VGH cells were treated with 100 μM Didox, or DFP or DFO for 4, 8, 24 and 48 h and analyzed for L- and H-ferritin content by an ELISA assay (Figure 6A,B) and for TfR1 by western blotting (Figure 6C).