AIM2 and infection: MAYV infection increased the levels of inflammasome-related mRNAs, including Aim2. However, infection did not activate the AIM2 inflammasome, since there was no alteration on secreted IL-1β levels using cells deficient in Aim2. Although MAYV infection induced an increase in steady-state Il1b transcript levels, priming with a TLR agonist prior to infection is needed for significant IL-1β secretion, in agreement with previously published literature with other viruses [45].