To test the tumor suppressive activity of Snora24 in a more aggressive liver cancer, we orthotopically injected primary tumor cells derived from Alb-cre;KrasG12D liver tumor (Xu et al., 2019), following CRISPR-Cas9 gene editing of the Snora24 loci using two distinct single guide RNA (sgRNA) targeting Snora24 (sgRNA-24) (Figure 2—figure supplement 2A), into the livers of wild-type mice. Here, SNORA24 is linked to neoplasm.