Snora24 reduction in this mouse model of liver cancer significantly decreased Snora24-guided pseudouridine modifications on 18S rRNA (Figure 2—figure supplement 2C) as shown using SCARLET (Site-specific Cleavage And Radioactive-labeling followed by Ligation-assisted Extraction and Thin-layer chromatography) (Li et al., 2015; Liu and Pan, 2015). The gene discussed is SNORA24; the disease is liver cancer.