To test the importance of these miRNAs in regulation of ZDHHC21 expression, we mimicked a miRNA environment in neuroblastoma cells by overexpressing the predicted miRNAs and found that transfection of miR-200a, miR-30a, and miR-30e all resulted in significant reductions, while transfection of miR-338-3p showed slightly increased ZDHHC21 expression (Fig. 6d). Here, ZDHHC21 is linked to neuroblastoma.