We next examined HOTAIRM1 expression in an independent glioma cohort (Grade II, n = 10, Grade III, n = 10, and Grade IV, n = 8), four non-neoplastic brain tissue specimens, five glioma cell lines, one normal astrocyte cell line, and three primary glioma cell lines [differentiated glioblastoma cells (DGCs)] derived from glioma patients. Here, HOTAIRM1 is linked to glioma.