In the present study, using RNA-sequencing (RNA-seq), we found that Notch1 up-regulated RNF187 expression in HCC cells, and Notch1-mediated HCC metastasis was attenuated when RNF187 was knocked down, whereas overexpression of RNF187 counteracted the inhibition of cancer progression mediated by Notch1 knockdown, suggesting that RNF187 is essential for Notch1-mediated HCC metastasis. Here, NOTCH1 is linked to hepatocellular carcinoma.