CD274 and neoplasm: PD-L1 IHC 22C3 pharmDx, PD-L1 IHC 28–8 pharmDx, and VENTANA SP263 assays showed similar PD-L1 prevalence for both TC and IC PD-L1 staining, with prevalence at the ≥25% cutoff ranging from 15.2 to 17.9% for TC staining, 21.5–25.1% for IC staining by IC area, and all at 0.6% for IC staining by tumor area (Additional files 2, 3, and 4).