CD274 and neoplasm: VENTANA SP142 showed similar prevalence versus the other three assays for ICs (22.7% staining for IC by IC area; 0.3% for IC staining by tumor area), but was less sensitive for PD-L1 staining on TCs (prevalence 6.3% at the ≥25% cutoff) (Additional files 2, 3, and 4).