For PCOS participants, an increase in SHBG (38.98± 11.02 nmol/L, P < 0.001) (Fig. 2.a) and a reduction in total testosterone and FAI were reported from baseline to week 24 of the study (total testosterone − 0.91 ± 0.33 nmol/L P = 0.006, FAI = − 4.47 ± 1.10, P < 0.001) (Fig. 2.b, c). This evidence concerns the gene SHBG and polycystic ovary syndrome.